Back to results
US 2015/0147349 A1Published application
Conformation-Stabilized TRAP Antigens
AssigneeChildren's Medical Center Corporation
InventorsTimothy A. Springer, Chafen Lu, Gaojie Song, Adem Koksal
Life of this patent
Estimating the term…
Abstract
This invention relates to compositions and methods for eliciting an immune response against a parasite of the genus Plasmodium in a mammal.
Claims22
A Plasmodium falciparum Thrombospondin-Related Anonymous Protein (TRAP) antigen, wherein the antigen sequence comprises one or more of the following (numbering relative to SEQ ID NO:5):
- (a) Mutation at Cysteine 55 to a non-cysteine amino acid;
- (b) Mutation of N-linked glycosylation sites;
- (c) Mutation of Ala-216/Asn-222 or Lys-224/Gln-78 to cysteine to create a TRAP that is stabilized in the open conformation;
- (d) Mutation of Asn-213/Ala-233, Ala-216/Phe-230, or Met-231/Gln-78 to cysteine to create a TRAP that is stabilized in the closed conformation;
- (e) Deletion of N-terminal and/or C-terminal residues to create a TRAP fragment that is stabilized in the closed conformation comprising V47-V238;
- (f) Deletion of N-terminal and/or C-terminal residues to create a TRAP fragment that is stabilized in the open conformation comprising V47-M231.
3 more elements
A Plasmodium vivax Thrombospondin-Related Anonymous Protein (TRAP) antigen, wherein the antigen sequence comprises one or more of the following (numbering relative to SEQ ID NO:6):
- (a) Mutation of N-linked glycosylation sites;
- (b) Mutation of Ser-212/Glu-218, Val-220/Ser-74 to cysteine to create a TRAP that is stabilized in the open conformation;
- (c) Mutation of Ser-212/Phe-226, Ile-223/Met-67, Ile-227/Ser-74 to cysteine to create a TRAP that is stabilized in the closed conformation;
- (d) Deletion of N-terminal and/or C-terminal residues to create a TRAP fragment that is stabilized in the closed conformation comprising amino acids V43-V234;
- and/or
- (e) Deletion of N-terminal and/or C-terminal residues to create a TRAP fragment that is stabilized in the open conformation comprising amino acids V43-I227.
3 more elements
-25. (canceled)
Dependencies are inferred from the claim text.