Methods and compositions of producing patient-specific multipotent neuronal stem cells
AssigneeInternational Stem Cell Corporation
InventorsRuslan Semechkin, Dmitry Isaev
Life of this patent
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Abstract
The present invention relates to the seminal discovery of compositions and a method of producing NSC obtained from stem cells derived from parthenogenically activated human oocytes (phNSC). The phNSC of the invention maintain proliferative and differentiation potential during cultivation and expansion.
Claims63
An isolated neuronal stem cell, wherein the cell is differentiated from a parthenogenetically activated oocyte.
A method for producing a neuronal stem cells by differentiating parthenogenetically derived human stem cells, the method comprising:
- a) growing parthenogenetically derived human stem cells on a feeder layer of fibroblast cells for at least 2 days;
- b) growing parthenogenetically derived human stem cells on a petri dish without fibroblast feeder layer for at least 1 day;
- c) culturing the cells in a neuronal induction media;
- d) obtaining a single cell suspension of the cells from (c);
- and
- e) culturing the single cells from step (d) on a petri dish with no fibroblast feeder layer in a neuronal proliferation media.
Isolated neuronal stem cells derived from parthenogenetically derived human stem cells using the method comprising:
- a) growing parthenogenetically derived human stem cells on a feeder layer of fibroblast cells for at least 2 days;
- b) growing parthenogenetically derived human stem cells on a petri dish with no fibroblast feeder layer for at least 1 day;
- c) culturing the cells in a neuronal induction media;
- d) obtaining a single cell suspension of the cells from (c);
- and
- e) culturing the single cells from step (d) on a petri dish with no fibroblast feeder layer in a neuronal proliferation media.
A method of treating a neurologic disorder using neuronal stem cells produced from parthenogenetically derived from oocytes.
A method of differentiating neuronal stem cells, the method comprising culturing neuronal stem cells in neuronal differentiation media.
A method for producing neuronal stem cells by differentiating parthenogenetically derived human stem cells, the method comprising:
- a) cultivation of human pluripotent stem cells in feeder-free conditions;
- b) exposure of said cells to neuronal induction medium;
- c) mechanical isolation of partially differentiated cells;
- and
- d) further expansion and maintenance of said cells until maturation.
Isolate neuronal stem cells derived from parthenogenetically derived human stem cells using the method comprising:
- a) cultivation of human pluripotent stem cells in feeder-free conditions;
- b) exposure of said cells to neuronal induction medium;
- c) mechanical isolation of partially differentiated cells;
- and
- d) further expansion and maintenance of said cells until maturation.
A method of treating a neurologic disorder using neuronal stem cells derived from parthenogenetically derived from oocytes.
A method of differentiating neuronal stem cells, the method comprising culturing neuronal stem cells in neuronal differentiation media.
Dependencies are inferred from the claim text.