Generation of functional and durable endothelial cells from human amniotic fluid-derived cells
AssigneeCornell University
InventorsShahin Rafii, Sina Y. Rabbany, Michael Ginsberg
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Abstract
This disclosure is directed to methods for reproducibly generating substantial amounts of endothelial cells from amniotic cells. The endothelial cells generated in accordance with the present methodology, as well as therapeutic methods utilizing these cells, are also disclosed.
Claims21
A method of generating endothelial cells, comprising
- obtaining amniotic cells;
- and
- culturing the amniotic cells under conditions where transcription factors ETV2, FLI1 and ERG are expressed in the amniotic cells in the presence of a TGFβ signaling inhibitor, thereby obtaining endothelial cells.
A substantially pure population of amniotic cell-derived ECs, wherein said ECs are characterized by expression of surface markers, VE-cadherin, CD31 and VEGFR2.
The substantially pure population of amniotic cell-derived ECs, wherein said ECs comprise an exogenously introduced nucleic acid encoding FLI1.
Dependencies are inferred from the claim text.