Mixed multifunctional metal affinity surfaces for reducing aggregate content in protein preparations

Abstract

Compositions for reducing the aggregate content of a protein preparation include a first substrate having a first surface-bound ligand possessing a metal affinity functionality and a second surface-bound ligand optionally provided on a second substrate and having an aggregate charge opposite to that of the metal affinity functionality of the first substrate, wherein the first surface-bound ligand and the second surface-bound ligand are positioned such that the protein preparation may contact both the first surface-bound ligand and the second surface-bound ligand simultaneously.

Dependencies are inferred from the claim text.