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US 2015/0144780 A1Published application
Method of MS/MS Mass Spectrometry
AssigneeMicromass UK Limited
InventorsJeffery Mark Brown, Martin Raymond Green, Steven Derek Pringle
Life of this patent
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Abstract
A method of mass spectrometry is disclosed comprising alternating between a first mode in which parent ions are mass analysed and a second mode in which the parent ions are subjected to Electron Capture Dissociation (“ECD”) at atmospheric pressure so as to produce fragment ions which are then mass analysed. The parent ions are associated with their fragment ions based on the times at which they were detected. This method enables parent ions to be associated with their fragment ions, even when the ECD fragmentation is performed at atmospheric pressure.
Claims26
A method of mass spectrometry comprising:
- (i) providing a plurality of different parent ions;
- (ii) mass analysing said parent ions so as to obtain first mass spectral data;
- (iii) subjecting said parent ions to Electron Capture Dissociation (“ECD”) or Electron Transfer Dissociation (“ETD”) at atmospheric pressure to produce fragment or product ions;
- (iv) mass analysing said fragment or product ions so as to obtain second mass spectral data;
- (v) wherein the parent ions are intermittently and repeatedly subjected to said ECD or ETD such that the method repeatedly alternates between steps (ii) and (iv);
- and
- (vi) associating parent ions detected in said first mass spectral data with fragment or product ions detected in said second mass spectral data.
4 more elements
A mass spectrometer comprising:
- an atmospheric pressure Electron Capture Dissociation (“ECD”) or Electron Transfer Dissociation (“ETD”) device;
- a mass analyser;
- and
- a control system arranged and adapted to:
- mass analyse parent ions so as to obtain first mass spectral data, in a first mode of operation;
- subject said parent ions to ECD or ETD at atmospheric pressure to produce fragment or product ions;
- mass analyse said fragment or product ions so as to obtain second mass spectral data, in a second mode of operation;
- intermittently and repeatedly subject said parent ions to said ECD or ETD so as to alternate between the first and second modes of operation;
- and
- associate parent ions detected in said first mass spectral data with fragment or product ions detected in said second mass spectral data.
7 more elements
A method of mass spectrometry comprising:
- providing a plurality of different parent ions;
- and
- performing at least one cycle comprising:
- (i) mass analysing said parent ions so as to obtain first mass spectral data;
- (ii) subjecting said parent ions to Electron Capture Dissociation (“ECD”) or Electron Transfer Dissociation (“ETD”) to produce fragment or product ions;
- and mass analysing said fragment or product ions so as to obtain second mass spectral data;
- (iii) subjecting said parent ions to ECD or ETD, thereby producing intermediate ions, wherein the intermediate ions are non-dissociated parent ions held together by non-covalent interactions or are charge-reduced parent ions that have not fragmented after being exposed to the ECD or ETD conditions;
- and subjecting said intermediate ions to a fragmentation technique other than ETD or ECD such that said intermediate ions fragment to form fragment ions;
- and mass analysing these fragment ions so as to obtain third mass spectral data;
- and
- (v) associating parent ions detected in said first mass spectral data with fragment or product ions detected in said second or third mass spectral data.
8 more elements
A mass spectrometer comprising:
- an Electron Capture Dissociation (“ECD”) or Electron Transfer Dissociation (“ETD”) device;
- a mass analyser;
- and
- a control system arranged and adapted to perform at least one cycle comprising:
- (i) mass analysing parent ions so as to obtain first mass spectral data;
- (ii) subjecting parent ions to ECD or ETD to produce fragment or product ions;
- and mass analysing said fragment and/or product ions so as to obtain second mass spectral data;
- (iii) subjecting parent ions to ECD or ETD, thereby producing intermediate ions, wherein the intermediate ions are non-dissociated parent ions held together by non-covalent interactions or are charge-reduced parent ions that have not fragmented after being exposed to the ECD or ETD conditions;
- and subjecting said intermediate ions to a fragmentation technique other than ETD or ECD such that said intermediate ions fragment to form fragment ions;
- and mass analysing these fragment ions so as to obtain third mass spectral data;
- and
- (v) associating parent ions detected in said first mass spectral data with fragment or product ions detected in said second or third mass spectral data.
9 more elements
A method of mass spectrometry comprising:
- generating a plurality of species of parent ions;
- varying the intensity profile of one or more species of parent ions as a function of time so that different species of parent ions are caused to have different intensity profiles as a function of time;
- subjecting said parent ions to Electron Capture Dissociation (“ECD”) or Electron Transfer Dissociation (“ETD”) at atmospheric pressure to produce fragment or product ions;
- mass analysing the fragment ions;
- and
- correlating the fragment ions with corresponding parent ions on the basis of the intensity profiles of said fragment ions and the intensity profiles of said parent ions.
3 more elements
A mass spectrometer comprising:
- means for generating a plurality of species of parent ions;
- means for varying the intensity profile of one or more species of parent ions as a function of time so that different species of parent ions are caused to have different intensity profiles as a function of time;
- means for subjecting said parent ions to Electron Capture Dissociation (“ECD”) or Electron Transfer Dissociation (“ETD”) at atmospheric pressure to produce fragment or product ions;
- means for mass analysing the fragment ions;
- and
- means for correlating the fragment ions with corresponding parent ions based on of the intensity profiles of said fragment ions and the intensity profiles of said parent ions.
3 more elements
Dependencies are inferred from the claim text.