Methods and materials for producing five carbon building blocks from proline
OwnerAdriana Leonora Botes, Alex Van Eck Conradieinventors
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Abstract
This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as D-proline.
Claims53
A method of producing a C5 building block selected from the group consisting of glutarate, 5-aminopentanoate, cadaverine, 5-hydroxpentanoate, and 1,5-pentanediol, said method comprising enzymatically synthesizing D-proline and enzymatically converting D-proline to said C5 building block in one or more enzymatic steps.
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A recombinant host comprising at least one exogenous nucleic acid encoding (i) a D-proline reductase, (ii) a 5-aminovalerate transaminase, and (iii) a dehydrogenase, said host producing glutarate or 5-hydroxypentanoate.
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A recombinant host comprising at least one exogenous nucleic acid encoding (i) a D-proline reductase, (ii) a carboxylate reductase, and (iii) a ω-transaminase, said host producing cadaverine.
A recombinant host comprising a) at least one exogenous nucleic acid encoding (i) a 5-aminovalerate transaminase, and (ii) a dehydrogenase, said host producing glutarate or 5-hydroxypentanoate or b) at least one exogenous nucleic acid encoding (iii) a carboxylate reductase and (iv) a ω-transaminase, said host producing cadaverine.
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Dependencies are inferred from the claim text.