Breeding method for two lines hybrid rice based on the rice osms4 gene mutant

Abstract

Provided is a breeding method for two lines hybrid rice based on the rice mutant osms4 (Oryza sativa male sterile 4), which was originally selected from the mutagenizing seeds of japonica cultivar 9522 with 60Co gamma ray, and the stable inheritance osms4 mutant was obtained after three generations backcrossing, which is regulated by a recessive single gene OsMS4. The invention is related to the usage of OsMS4 gene (also known as Carbon Starved Anther, the CSA gene), osms4 mutant can be used as male sterile line under short day condition in both japonica and indica background, which grows abnormal anther with defection during second mitosis stage and produces completely sterile pollen. While under the long day condition, the osms4 mutant can be used as maintainer liner and produces normal pollen.

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