Use of disulfide bonds to form a reversible and reusable coating for nanofluidic devices
AssigneeInternational Business Machines Corporation
InventorsNatalie Gunn, Jose M. Lobez Comeras, Priscilla Rogers, John Wagner
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Abstract
A reusable coating for a nanopore structure is disclosed herein. A nanopore structure includes a substrate comprising a nanochannel and a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel. The chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel. Methods for making and using the reusable coating are also disclosed.
Claims10
A method for using a re-usable coating within a nanopore structure, the method comprising:
- forming a monolayer of a chemical compound on a surface of a nanochannel within the nanopore structure;
- reacting an analyte binding compound with the chemical compound of the monolayer to form a reversible bond, wherein the analyte binding compound comprises a functional group forming the reversible bond and an analyte binding group;
- measuring one or more electrical signals for an event, the event being a translocation event, a binding event, or both the translocation event and the binding event;
- severing the reversible bond;
- and
- flushing the analyte binding compound from the nanochannel.
A method for making a reversible analyte-binding coating in a nanopore structure, the method comprising:
- coating a surface of a nanochannel with a chemical compound comprising a reactive functional group;
- disposing an analyte binding compound onto a surface of the nanochannel to form a reversible bond with the reactive functional group, the analyte binding compound comprising an analyte binding group;
- introducing an analyte into the nanochannel, wherein the analyte binds to the analyte binding group;
- and
- introducing a severing agent into the nanochannel to reform the reactive functional group.
Dependencies are inferred from the claim text.