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US 2015/0104869 A1Published application
Small Activating RNA Molecules and Methods of Use
AssigneeThe Regents of the University of California
InventorsLongcheng Li, Rajvir Dahiya
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Abstract
The present invention provides compositions, pharmaceutical preparations, kits and methods for increasing expression of a gene product in a cell by contacting the cell with a small activating RNA (saRNA) molecule comprising a ribonucleic strand that is complementary to a non-coding nucleic acid sequence of the gene.
Claims16
-73. (canceled)
A method to increase expression of a gene comprising:
- introducing a small activating RNA (saRNA) molecule into a mammalian cell in an amount sufficient to increase expression of a gene, wherein the saRNA molecule comprises at least a first ribonucleic acid strand comprising:
- a 5′ region of complementarity to a promoter sequence of the gene;
- and
- a 3′ terminal region of at least one nucleotide non-complementary to the promoter sequence,
- wherein the region of complementarity comprises between about 20 to about 25 base pairs,
- wherein the introducing results in an increase in expression of the gene.
3 more elements
An isolated composition comprising,
- a small activating RNA (saRNA) molecule comprising at least a first ribonucleic acid strand comprising a region of complementarity to a promoter sequence of a vascular endothelial growth factor (VEGF) gene sufficient to activate transcription of the VEGF gene, wherein the region of complementarity comprises between about 20 to about 25 base pairs.
Dependencies are inferred from the claim text.