US 2015/0104851 A1 · METHOD FOR THE BIOCATALYTIC CYCLIZATION OF TERPENES AND CYCLASE MUTANTS EMPLOYABLE THEREIN

Abstract

The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.

Claims17

Dependent claims

An enzyme mutant with cyclase activity which comprises an amino acid sequence selected from the group consisting of the amino acid sequences of SEQ ID NO:

2 to 326 or a partial sequence thereof, wherein said enzyme mutant is a mutant of a wildtype enzyme and catalyzes at least the cyclization of a citronellal isomer to at least one isopulegol isomer.

Dependencies are inferred from the claim text.

Facts

Application

14/560,263Filed 4 Dec 2014

Priority

17 Nov 2010

Published

16 Apr 2015

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C12N 9/88
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: lyases
C12N 9/90
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: isomerases
C12P 5/007
Preparation of hydrocarbons or halogenated hydrocarbons: containing one or more isoprene units, i.e. terpenes
C12P 7/02
Preparation of oxygen-containing organic compounds: containing a hydroxy group

Family

Application61/414,434Us provisional application · filed 17 Nov 2010

Application61/499,228Us provisional application · filed 21 Jun 2011

Application61/540,028Us provisional application · filed 28 Sep 2011

Application13/297,798Division · filed 16 Nov 2011

Application14/560,263Division

US 2012/0237991 A1Published application

US 8,932,839 B2Related patent

US 9,447,404 B2Related patent

US 2015/0104851 A1This patent

From the US filing history. Foreign family members aren’t listed.

Method for the biocatalytic cyclization of terpenes and cyclase mutants employable therein

Life of this patent

Method for the biocatalytic cyclization of terpenes and cyclase mutants employable therein

Life of this patent

Abstract

Claims17