Culture Medium and Methods for Producing Alginate From Stable Mucoid Strains of Pseudomonas Aeruginosa
AssigneeProgenesis Technologies, LLC
InventorsHongwei David Yu, Richard M. Niles, Xin Wang, Kristy Dawn Dillon
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Abstract
A specialized culture medium for the promotion of alginate production by stable mucoid Pseudomonas aeruginosa bacterial strains and methods for the production and purification of industrial, commercial, and pharmaceutical grade alginate from bacteriological sources are provided herein. Alginate produced using the media and methods disclosed herein is structurally uniform and substantially free of bacterial cell contaminants, including endotoxin.
Claims20
A liquid culture medium for the promotion of alginate production by Pseudomonas aeruginosa (P. aeruginosa) bacterial cells belonging to a strain having a stable mucoid phenotype, the culture medium comprising:
- P. aeruginosa bacterial cells belonging to a strain having a stable mucoid phenotype;
- about 1% (w/v) enzymatic digest of animal protein;
- about 1% (w/v) pancreatic digest of gelatin;
- K2SO4;
- MgCl2;
- and
- from about 5% (v/v) to about 14% (v/v) glycerol, wherein “about” refers to a variation of ±20% of a value.
A liquid culture medium for the promotion of alginate production by Pseudomonas aeruginosa (P. aeruginosa) bacterial cells belonging to a strain having a stable mucoid phenotype, the culture medium comprising:
- P. aeruginosa bacterial cells belonging to a strain having a stable mucoid phenotype;
- about 1% (w/v) enzymatic digest of animal protein;
- about 1% (w/v) pancreatic digest of gelatin;
- about 2% (w/v) K2SO4;
- about 0.5% (w/v) MgCl2;
- about 10% (v/v) glycerol;
- and
- about 25 mg/liter triclosan,
- wherein “about” refers to a variation of ±20% of a value.
Dependencies are inferred from the claim text.