Assay for trichomonas vaginalis by amplification and detection of trichomonas vaginalis ap65-1 gene
AssigneeBecton, Dickinson and Company
InventorRay A. Mc Millian
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Abstract
A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.
Claims17
A probe set for the detection of the AP65-1 gene of Trichomonas vaginalis comprising four primers and one probe wherein the four primers and one probe each have an oligonucleotide primer sequence wherein the four oligonucleotide primer sequences comprise SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9 and SEQ ID NO:10 and the one probe oligonucleotide sequence comprises SEQ ID NO:11.
A kit comprising:
- a probe set for the amplification detection of Trichomonas vaginalis that contains a AP65-1 gene (SEQ ID NO:1)
- a) one or more primers comprising oligonucleotide
- sequences that bind to the highly conserved region of the SEQ ID NO:1;
- b) at least one detector comprising an oligonucleotide sequence that binds to a region of SEQ ID NO:1.
Dependencies are inferred from the claim text.