Methods and compositions for exosome isolation
AssigneeLife Technologies Corporation
InventorsAlexander VLASSOV, Mu LI, Emily ZERINGER, Richard CONRAD
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Abstract
Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
Claims31
A method for the isolation of exosomes from a biological fluid sample comprising:
- a) adding a volume-excluding polymer to the biological fluid sample,
- b) incubating the biological fluid sample with the volume-excluding polymer;
- and
- c) isolating the precipitated exosomes from the biological fluid sample.
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A method for the isolation of exosomes from a biological tissue sample comprising:
- a) lysing the biological tissue sample,
- b) clarifying the lysed sample
- c) adding a volume-excluding polymer to the clarified sample,
- d) incubating the clarified sample with the volume-excluding polymer, and
- e) isolating the precipitated exosomes.
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A kit for the isolation of exosomes comprising:
- a) one or more vessels containing one or more volume-excluding polymers, one or more buffers or one or more solutions for performing density gradient centrifugation of exosomes,
- b) one or more vessels containing antibodies or other ligands which bind a surface marker exposed on the surface of the exosome, and
- c) one or more solid supports which bind directly or indirectly to the exosomes.
Dependencies are inferred from the claim text.