Back to results
US 2015/0104797 A1Published application
Composite visible colorant and method for quantitative amplification
AssigneeBio-Rad Laboratories, Inc.
InventorRongdian Fu
Life of this patent
Estimating the term…
Abstract
Methods and compositions for using colorants with real-time amplification reactions and normalization passive dyes are provided.
Claims17
A composite colorant formulation comprising:
- a visually-detectable dye;
- and
- an internal reference molecule comprising a first fluorophore, which first fluorophore upon excitation emits energy at a specific wavelength range;
- wherein:
- the visually-detectable dye partially absorbs energy at the specific wavelength range;
- and
- the formulation does not comprise sufficient components for target nucleic acid amplification if added to a target nucleic acid and primers.
4 more elements
The composite colorant formulation of any of the above claims, wherein the formulation lacks a polymerase, nucleotides, or divalent cations.
Dependencies are inferred from the claim text.