Reverse Transcriptase with Enhanced Properties
AssigneeNew England Biolabs, Inc.
InventorsYinhua Zhang, Thomas C. Evans, JR.
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Abstract
Compositions and methods are provided for improved reverse transcriptases and their uses in reverse transcription where the improvement may include increased temperature, increased salt, increased activity and/or increased dUTP tolerance.
Claims31
A non-naturally occurring polypeptide comprising an amino acid sequence at least 96% identical to non-naturally occurring SEQ ID NO:2.
A method of reverse transcribing RNA, the method comprising exposing the RNA to a non-naturally occurring polypeptide comprising an amino acid sequence at least 96% identical to non-naturally occurring SEQ ID NO:2 wherein the composition comprises primers, a buffer, and deoxyribonucleotides, thereby to reverse transcribe the RNA to produce a complementary strand of DNA.
Dependencies are inferred from the claim text.