Cell analysis method, cell analyzer and sample screening method
AssigneeSysmex Corporation
InventorsKei SHIRASUNA, Ryuichiro EBI, Shigeki ABE
Life of this patent
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Abstract
Disclosed is a cell analysis method comprising: extracting target cells from a population of cells derived from an epithelial tissue on the basis of N/C ratio representing a relative size of a nucleus to a cytoplasm; classifying the target cells into at least a first group and a second group by difference of amount of DNA; and evaluating a pathology of the epithelial tissue by comparing a ratio of numbers of cells between the first and second groups with a threshold; wherein the threshold varies according to a proportion of the target cells in the population.
Claims20
A cell analysis method comprising:
- extracting target cells from a population of cells derived from an epithelial tissue on the basis of N/C ratio representing a relative size of a nucleus to a cytoplasm;
- classifying the target cells into at least a first group and a second group by difference of amount of DNA;
- and
- evaluating a pathology of the epithelial tissue by comparing a ratio of numbers of cells between the first and second groups with a threshold;
- wherein the threshold varies according to a proportion of the target cells in the population.
A cell analyzer comprising:
- a measurement apparatus comprising a cytometer;
- and
- a data processing device connected to the cytometer, and comprising a processor programmed to process data of cells obtained from the cytometer by:
- extracting target cells from a population of cells derived from an epithelial tissue on the basis of N/C ratio representing a relative size of a nucleus to a cytoplasm;
- determining a threshold according to a proportion of the target cells in the population;
- classifying the target cells into at least a first group and a second group by difference of amount of DNA;
- and
- evaluating a pathology of the epithelial tissue by comparing a ratio of numbers of cells between the first and second groups with the threshold determined.
A sample screening method comprising:
- quantifying target cells in a measurement specimen prepared from a sample of epithelial tissue on the basis of N/C ratio representing a relative size of a nucleus to a cytoplasm;
- evaluating on the basis of quantity of the target cells whether the sample was appropriately collected from a subject;
- screening on the basis of amounts of DNA of the target cells the sample into groups of a cancer-suspected sample and other if the sample was appropriately collected;
- avoiding the screening if the collection of sample was inappropriate.
Dependencies are inferred from the claim text.