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US 2015/0104441 A1Published application
Identification of disease-driving antigens
AssigneeOslo Universitetssykehus Hf
InventorsJohanna Olweus, Trygve Holmøy, Andreas Lossius
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Abstract
Provided herein is technology relating to treating disease and particularly, but not exclusively, to methods for identifying disease-related antigens by assessing T cell receptor gene frequencies in diseased subjects.
Claims20
A method for identifying a disease-related antigen, the method comprising:
- a) sequencing a set of T cell receptor genes from a disease-related sample from a subject having a disease;
- b) sequencing a set of T cell receptor genes from a control sample;
- c) comparing the T cell receptor gene frequencies of the set of T cell receptor genes from the disease-related sample with the T cell receptor gene frequencies of the set of T cell receptor genes from the control sample to identify T cell receptor genes enriched in the disease-related sample,
- wherein the T cell receptor genes enriched in the disease-related sample identify a disease-related antigen associated with the disease.
1 more element
A method of identifying a disease-related antigen, the method comprising:
- a) contacting a control sample from a subject diagnosed with an inflammatory or an autoimmune disease with a test antigen and a control antigen;
- b) isolating T cells from the control sample that respond to said test antigen to provide control sample test-antigen responsive T cells and isolating T cells from the control sample that respond to said control antigen to provide control sample control-antigen responsive T cells;
- c) sequencing T-cell receptor genes from said control sample test-antigen responsive T cells to provide control sample test-antigen responsive gene sequences and sequencing T-cell receptor genes from said control sample control-antigen responsive T cells to provide control sample control-antigen responsive gene sequences;
- d) isolating T cells from the disease-related sample to provide disease-related T cells;
- e) sequencing T-cell receptor genes from said disease-related T cells to provide disease related gene sequences;
- and
- f) comparing the frequency of said control sample test-antigen responsive gene sequences and control sample control-antigen responsive gene sequences with the frequency of said disease-related gene sequences,
- wherein the gene sequences enriched in the disease-related sample identify a disease-related antigen associated with the disease.
5 more elements
Dependencies are inferred from the claim text.