Cellular production of glucaric acid
AssigneeMassachusetts Institute of Technology
InventorsTAE SEOK MOON, Sang-Hwal Yoon, Kristala Lanett Jones Prather
Life of this patent
Estimating the term…
Abstract
The invention relates to the production of glucuronic and glucaric acid in cells through recombinant expression of myo-inositol 1-phosphate synthase, myo-inositol oxygenase and uronate dehydrogenase. Cloning and characterization of the gene encoding uronate dehydrogenase is also disclosed.
Claims30
A cell that recombinantly expresses a gene encoding uronate dehydrogenase and recombinantly expresses a gene encoding myo-inositol oxygenase.
-6. (canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A genetically modified microorganism that comprises one or more recombinant nucleic acid molecules encoding uronate dehydrogenase, myo-inositol oxygenase and myo-inositol 1-phosphate synthase.
A method for producing glucaric acid, the method comprising genetically modifying a cell to recombinantly express at least one of:
- uronate dehydrogenase, myo-inositol oxygenase and myo-inositol 1-phosphate synthase, culturing a population of said cells, and collecting glucaric acid from the population of cells that have been genetically modified to produce glucaric acid.
-33. (canceled)
(canceled)
(canceled)
43-74. (canceled)
Dependencies are inferred from the claim text.