Tissue-engineered heart valve for transcatheter repair
AssigneeCytograft Tissue Engineering, Inc.
InventorsNicolas L'Heureux, Todd N. McAllister, Nathalie Dusserre
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Abstract
A single-layer tissue sheet having a puncture strength of 2 kgf to 6 kgf. A valve, such as a heart valve, made of one or more leaflets formed from a single-layer tissue sheet. A method of making a tissue sheet having a puncture strength of 2 kgf to 5 kgf. The ultra-strong tissue sheets described herein have very long culture times, such as in excess of 20 weeks. Valves that comprise one or more leaflets made from the ultra-strong tissue sheets described herein may be delivered via trans-cathete aortic valve implantation.
Claims16
A single-layer tissue sheet having a puncture strength of 2 kgf to 6 kgf.
A valve implant comprising:
- a tubular structure;
- and
- two or more leaflets, connected to the tubular structure,
- wherein the two or more leaflets each comprise a single-layer tissue-engineered sheet having a puncture strength of 2 kgf to 6 kgf.
The method of making a tissue-engineered valve implant, the method comprising:
- growing a tissue sheet having a puncture strength that exceeds 2 kgf;
- cutting two or more leaflets from the tissue sheet;
- and
- attaching the two or more leaflets to a stent, thereby creating a valve implant.
A method of repairing a heart valve, the method comprising:
- collapsing a tissue-engineered heart valve on to a transcatheter;
- and
- delivering the tissue-engineered heart valve into place via a catheter,
- wherein the tissue engineered heart valve comprises two or more leaflets made form tissue-engineered sheets.
A method of making a single layer tissue sheet, the method comprising:
- culturing a population of cells, in a culture dish with one or more tissue control rods, for a period exceeding 20 weeks, to make a sheet comprising the cells and extracellular matrix produced by the cells;
- and
- during the period and after two weeks, seeding the sheet with a suspension of cells from the same cell line as the population of cells,
- wherein the seeding is carried out 2-6 times.
Dependencies are inferred from the claim text.