Loading patent…
AssigneeZeochem LLC
InventorsNicola Forrer, Mandy Erdmann, David Gétaz, Massimo Morbidelle, Susanna Bernardi, Rushd Khalaf
Estimating the term…
A material for reverse phase chromatography comprises surface modifying apolar and charged groups bound to a solid support, said charged groups being present in amounts of about 0.25 to about 22% of the surface modifying groups, or in amounts of about 0.01 μmol/m2 to 0.8 μmol/m2 referred to the surface of the solid support for a material with a total amount of surface modifying groups of 3.6 μmol/m2. Such material and suitable purification conditions for active pharmaceutical ingredients (APIs) like peptides can be evaluated by (a) determining the isoelectric point (pI) of the API of interest, (b) choosing a pH in a range where the solid phase material is stable, (c) determining the difference pI-pH and (d) if the difference pI-pH is positive, choosing an anion exchange (AIEX) material, or if the difference pI-pH is negative, choosing an cation exchange (CIEX) material.
A material for reverse phase chromatography comprising surface modifying groups bound to a solid support, wherein said surface modifying groups are not horizontally polymerized, said surface modifying groups comprising apolar uncharged groups and polar groups, and wherein said polar groups being charged groups, said charged groups being either positively charged or negatively charged, and said charged groups being present in amounts of about 0.25 to about 22% of the surface modifying groups, or in amounts of about 0.01 μmol/m2 to 0.8 μmol/m2 referred to the surface of the solid support for a material with a total amount of surface modifying groups of 3.6 μmol/m2.
A method comprising separating a protein from at least one other active pharmaceutical ingredient (API) by subjecting a mixture of the protein and the at least one other API in an eluent system having a pH to a material, the material comprising surface modifying groups bound to a solid support, said surface modifying groups comprising apolar uncharged groups and polar groups, wherein said polar groups are positively charged groups, and wherein the isoelectric point (pI) of the protein>the pH of the eluent system.
A method comprising separating a protein from at least one other active pharmaceutical ingredient (API) by subjecting a mixture of the protein and the at least one other API in an eluent system having a pH to a material, the material comprising surface modifying groups bound to a solid support, said surface modifying groups comprising apolar uncharged groups and polar groups, wherein said polar groups are negatively charged groups, and wherein the isoelectric point (pI) of the protein<the pH of the eluent system.
Dependencies are inferred from the claim text.