Composition for Diagnosing Sepsis, and Method and Kit Therefor

An information offering method for diagnosing sepsis comprising:

• (a) separating a DNA from a clinical specimen;
• (b) performing polymerase chain reaction (PCR) amplification of a 16s rRNA gene, an ITS (internal transcribed sequence) gene, a Nuc (heat-stable DNA nuclease) gene, a Cps gene (S. pneumoniae encoding biosyntheiss of capsular polysaccharide), a MecA gene (gene encoding methicillin resistance in saphylococci), an invA (invasion A) gene for detecting salmonella, an ipaH (invasion plasmid antigen) for detecting Shigella, and genetic fragments of a Van (Vancomycin resistance protein) A and a Van (Vancomycin resistance protein) B from the DNA by using respective primers;
• and
• (c) forming a PCR-reverse blot hybrid with a solid support upon which an oligomer probe for detecting the 16s rRNA gene for distinguishing gram-positive bacteria and gram-negative bacteria, an oligomer probe for detecting the ITS (internal transcribed sequence) gene for identifying a fungus, an oligomer probe for detecting the MecA gene for checking whether or not MRSA has antibiotic resistance, a probe for detecting the Nuc gene specific to S. aureus, a probe for detecting the Cps gene specific to S. pneumoniae, and a probe for detecting the invA gene for detecting salmonella, a probe for detecting the ipaH for detecting Shigella, and an oligomer probe for detecting the VanA and the VanB for checking whether or not VRE have antibiotic resistance are attached, and an amplified product obtained from the step (b).