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US 2015/0086988 A1Published application
Compositions and methods of selective nucleic acid isolation
AssigneeApplied Biosystems, LLC
InventorsDouglas BOST, Lawrence GREENFIELD
Life of this patent
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Abstract
The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
Claims30
A method for isolating DNA and RNA from a biological sample comprising:
- selectively binding DNA to a first solid phase by contacting the biological sample with the first solid phase under conditions which selectively bind DNA;
- separating the first solid phase with the bound DNA from a first unbound portion of the biological sample;
- isolating the DNA from the first solid phase;
- and
- isolating RNA from the first unbound portion of the biological sample, wherein the isolating of the RNA from the first unbound portion of the biological sample comprises:
- exposing the first unbound portion of the biological sample to a second solid phase under conditions which bind RNA to the second solid phase, wherein the conditions which bind RNA to the second solid phase comprise a neutral or acidic pH;
- separating the second solid phase with bound RNA from a second unbound portion of the biological sample;
- and
- isolating the RNA from the second solid phase.
6 more elements
4-6. (canceled)
A method of isolating nucleic acid from a biological sample comprising:
- binding nucleic acid to a first solid phase by contacting the biological sample with the first solid phase under conditions which bind both RNA and DNA;
- separating the first solid phase with bound nucleic acid from a first unbound portion of the biological sample;
- eluting RNA from the first solid phase with bound nucleic acid under conditions which selectively bind DNA;
- removing the first solid phase with bound DNA from a first eluate;
- and
- isolating the DNA from the first solid phase.
3 more elements
16-18. (canceled)
28-30. (canceled)
34-35. (canceled)
A method of identifying nucleic acid in a biological sample comprising:
- binding nucleic acid to a first solid phase by contacting the biological sample with the first solid phase under conditions which bind both DNA and RNA;
- separating the first solid phase with bound nucleic acid from a first unbound portion of the biological sample;
- eluting RNA from the first solid phase with bound nucleic acid under conditions which selectively bind DNA;
- removing the first solid phase with bound DNA from a first eluate;
- and identifying the DNA bound to the first solid phase.
2 more elements
Dependencies are inferred from the claim text.