Individualized cancer therapy

A method for treating cancer, comprising:

• obtaining a specimen of cancer tissue and normal tissue from a patient;
• extracting total protein and RNA from the cancer tissue and normal tissue;
• obtaining a protein expression profile of the cancer tissue and normal tissue;
• identifying over-expressed proteins in the cancer tissue;
• identifying at least one prioritized protein target by assessing connectivity of each over-expressed protein;
• designing a first RNA interference expression cassette to modulate the expression of at least one gene encoding the prioritized target protein;
• incorporating the first cassette into a first delivery vehicle;
• and
• providing a patient with an effective amount of the first delivery vehicle sufficient to treat the cancer.

A method for treating cancer, comprising:

• obtaining a specimen of cancer tissue and normal tissue from a patient;
• extracting total protein and RNA from the cancer tissue and normal tissue;
• obtaining a protein expression profile of the cancer tissue and normal tissue;
• identifying over-expressed proteins in the cancer tissue;
• comparing the protein expression profile to a gene expression profile;
• identifying at least one prioritized protein target by assessing connectivity of each said over-expressed protein to other cancer-related or stimulatory proteins;
• designing a first RNA interference expression cassette to modulate the expression of at least one gene encoding the prioritized target protein;
• designing a first RNA interference expression cassette to modulate the expression of at least one gene encoding a protein of higher priority in the signaling pathway in which the first protein is a component;
• incorporating the first cassette into a first delivery vehicle;
• providing a patient with an effective amount of the first delivery vehicle;
• extracting total protein and RNA from the treated cancer tissue;
• identifying over-expressed proteins in the treated cancer tissue;
• designing a second RNA interference expression cassette to modulate the expression of a second prioritized protein in the treated tissue;
• incorporating the second cassette into a second delivery vehicle;
• providing the previously treated patient with an effective amount of the second delivery vehicle;
• identifying a novel protein signal following prior treatment with protein specific knockdown;
• identifying a gene mutation provided by gene sequencing/microarray on assessment of other protein signals;
• and
• identifying of a novel protein signal as a result of determination of the gene mutation and assessment of other protein signals.