Methods and compositions for expression of transgenes in plants
AssigneeMonsanto Technology LLC
InventorsLarry Gilbertson, Jason Ward, Jianping Xu, Jon Lamb
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Abstract
Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.
Claims28
A method for expressing at least 10 transgenes in a plant comprising:
- expressing the at least 10 transgenes in the plant, wherein the transgenes are arranged in tandem in a single locus of the plant, the at least 10 transgenes being operably linked to promoter sequences, wherein the at least 10 transgenes are transformed into the single locus as a single transformation event with a transformation construct comprising the at least 10 transgenes, and
- wherein each of the at least 10 transgenes are expressed at levels sufficient to confer a selectable or screenable marker phenotype or a trait of agronomic interest to the plant.
9-10. (canceled)
12-13. (canceled)
The method of claim 28, wherein expression of at least two of the transgenes of the plant is enhanced relative to expression of the same transgenes in an otherwise isogenic plant having fewer than 10 transgenes.
The method of claim 28, wherein at least one of the transgenes transformed into the single locus confers a trait of agronomic interest to the transgenic plant.
The method of claim 28, wherein the single locus of the transgenic plant comprises at least one transgene that confers herbicide tolerance and at least one transgene that confers insect resistance.
The method of claim 28, wherein the transgenic plant is selected from the group consisting of wheat, maize, rye, rice, corn, oat, barley, turfgrass, sorghum, millet, sugarcane, tobacco, tomato, potato, soybean, cotton, canola, sunflower and alfalfa.
21-22. (canceled)
A method of making a transgenic plant comprising:
- transforming via Agrobacterium-mediated transformation at least 10 transgenes into a single locus of an explant, the transformation into the single locus occurring as a single transformation event with a transformation construct comprising the at least 10 transgenes, wherein the at least 10 transgenes are operatively linked to promoter sequences;
- and
- regenerating a transgenic plant from the transformed explant, the transgenic plant having the at least 10 transgenes arranged in tandem in the single locus, wherein each of the at least 10 transgenes are expressed at levels sufficient to confer a selectable or screenable marker phenotype or a trait of agronomic interest to the transgenic plant.
Dependencies are inferred from the claim text.