Real time pcr detection of m. tuberculosis resistant/susceptible to rifampicin and/or isoniazid

A method for the detection of at least one rifampicin and/or isoniazid Mycobacterium tuberculosis bacterium in a biological sample, wherein the method comprises:

• (a) isolating nucleic acids from the biological sample and optionally performing a reverse transcription, and
• (b) conducting real-time PCR, wherein primer sets selected from the sequences depicted in SEQ ID NOs:
• 1 and 2 and/or SEQ ID NOs:
• 4 and 5 and/or SEQ ID NOs:
• 7 and 8 or complements thereof or sequences having at least 90 or 95% identity to any of SEQ ID NOs:
• 1, 2, 4, 5, 7 or 8 or complements thereof are used, and an oligonucleotide probe is used, said probe specifically recognizing nucleotide polymorphisms in at least one of the following genes:
• the rpoB gene, the katG gene and the inhA gene of Mycobacterium tuberculosis and wherein at least one probe is selected from the sequences depicted in SEQ ID NOs:
• 3, 6 and 9 or complements or sequences having at least 90, or 95% identity to any of SEQ ID NOs:
• 3, 6 or 9 or complements thereof.