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US 2015/0031558 A1Published application
Methods, compositions and systems for sample deposition
AssigneeLife Technologies Corporation
InventorsTodd REARICK, Jessica Lynn REED, Jason GIOIA, Devin DRESSMAN, Nicholas HAPSHE, Brian REED, John Andrew SHERIDAN
Life of this patent
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Abstract
Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.
Claims20
A method of analyzing a biomolecule, the method comprising:
- coupling the biomolecule to a particle in a sample solution;
- applying at least a portion of the sample solution to a device including a flow cell defined between a cap and an array of wells, the array of wells providing access to a plurality of sensors;
- centrifuging the device while the sample solution is present in the flow cell, the device centrifuged at an angle relative to a radial vector in a range of 0° to 90°;
- and
- coupling the device to a measurement system.
2 more elements
A method of analyzing a nucleic acid, the method comprising:
- applying at least a portion of a sample solution to an array, the sample solution comprising a particle substrate coupled to a plurality of copies of the nucleic acid;
- centrifuging the array while in the presence of the at least a portion of the sample, the array including a plurality of wells in cooperative association with a plurality of sensors, the array disposed at an angle relative to a radial vector in a range of 0° to 90° during centrifuging, the particle substrate depositing into a well of the plurality of wells;
- and
- repeating applying and centrifuging.
1 more element
A method of analyzing a biomolecule, the method comprising:
- contacting a substrate with a sample solution comprising a population of different particles coupled to a different biomolecules;
- and
- centrifuging the substrate and the sample solution such that at least a subpopulation of the different particles randomly associates onto sites on the substrate.
Dependencies are inferred from the claim text.