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US 2015/0031555 A1Published application
Method for correction of bias in multiplexed amplification
AssigneeGigaGen, Inc.
InventorsDavid Scott Johnson, Andrea Loehr
Life of this patent
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Abstract
This invention relates a method to correct for bias inherent to multiplexed sequence amplification. The resulting corrected data is a much more accurate representation of true quantities than unprocessed data.
Claims17
A method for preparing a series of mathematical functions for correction of bias in amplification of a plurality of immune related sequences which comprises:
- a. amplifying a first mixture comprising at least two different immune related sequences at known concentrations;
- b. amplifying a second mixture comprising the immune related sequences of step (a) wherein the sequences are present at different concentrations than the first mixture;
- c. measuring sequence counts for the first and second amplified mixtures of immune related sequences;
- d. generating a plurality of mathematical functions for correction of bias that model relationships between concentrations and measured sequence counts;
- e. assembling the mathematical functions of step (d) to generate the series of mathematical functions useful to correct amplification bias.
2 more elements
A computer-implemented method for correcting bias in a plurality of immune related sequences which comprises:
- a. obtaining a plurality of measurements of levels of amplified immune related sequences from an unknown sample;
- and
- b. using an assembled series of mathematical functions and the measurements from step (a) to correct amplification bias in amplified immune related sequences from the unknown sample.
A system for correcting bias in amplified immune related sequences which comprises:
- a. an assembler module comprising an assembled series of mathematical functions useful to correct amplification bias in a plurality of immune related sequences;
- and
- b. a calculating module that corrects bias in amplification of immune related sequences using the assembled series of mathematical functions and measurements of levels of amplified immune related sequences from an unknown sample.
Dependencies are inferred from the claim text.