Human antibodies and proteins
AssigneeAntitope Limited
InventorsTimothy David Jones, Matthew Paul Baker
Life of this patent
Estimating the term…
Abstract
The present invention provides composite proteins, including antibodies, which show reduced immunogenicity. In particular, composite antibodies for use in humans are provided, in particular antibodies which have been modified to remove one or more T-cell epitopes. Methods for generating such proteins are also provided.
Claims26
A modified antibody or antigen-binding fragment thereof wherein the heavy and light chain variable regions of the modified antibody or antigen-binding fragment are each composed of two or more segments of amino acid sequence from one or more other antibodies or antigen-binding fragments, whereby the segments are neither whole CDRs nor framework regions.
2-36. (canceled)
A method for screening composite antibody variable regions comprising:
- generating a library of genes encoding composite antibody variable regions derived from multiple segments of amino acid sequence of 2 to 31 amino acids long from other antibodies or antigen-binding fragments, wherein the multiple segments are neither whole CDRs nor whole framework regions;
- screening the composite antibody variable regions to avoid T cell epitopes;
- and
- expressing at least a portion of the library and screening the expressed antibody variable regions for binding to one or more antigens of interest.
A method for producing copies of a composite antibody variable region of interest comprising:
- generating a library of genes encoding composite antibody variable regions derived from multiple segments of amino acid sequence of 2 to 31 amino acids long from other antibodies or antigen-binding fragments, wherein the multiple segments are neither whole CDRs nor whole framework regions;
- screening the composite antibody variable regions to avoid T cell epitopes;
- expressing at least a portion of the library and screening the expressed antibody variable regions for binding to one or more antigens of interest;
- identifying an antibody variable region of interest;
- and
- producing additional copies of the identified antibody variable region of interest.
A method for screening composite antibody variable regions comprising:
- generating a library of genes encoding composite antibody variable regions derived from multiple segments of amino acid sequence of 2 to 31 amino acids long from other antibodies or antigen-binding fragments, wherein the multiple segments are neither whole CDRs nor whole framework regions;
- and
- expressing at least a portion of the library and screening the expressed antibody variable regions for binding to one or more antigens of interest.
A method for producing copies of a composite antibody variable region of interest comprising:
- generating a library of genes encoding composite antibody variable regions derived from multiple segments of amino acid sequence of 2 to 31 amino acids long from other antibodies or antigen-binding fragments, wherein the multiple segments are neither whole CDRs nor whole framework regions;
- expressing at least a portion of the library and screening the expressed antibody variable regions for binding to one or more antigens of interest;
- identifying an antibody variable region of interest;
- and
- producing additional copies of the identified antibody variable region of interest.
Dependencies are inferred from the claim text.