Compositions and methods for chemical reporter vectors
AssigneeThe Regents of the University of Colorado, a body corporate
InventorsRyan T. Gill, Michael D. Lynch, Tirzah Y. Mills
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Abstract
Embodiments of the present invention concerns methods and compositions for the construction of a series of vectors containing a chemical sensing module to assess the production of a chemical compound by a microorganism.
Claims13
A method comprising:
- a) obtaining a chemical reporter having a set of genetic elements comprising, a chemical sensor module;
- a regulated promoter;
- and a selectable marker;
- and
- b) using the chemical reporter with a multi-scale analysis library enrichment method (SCALEs) to identify a genetic element capable of altering the expression of a non-selectable phenotype.
A method comprising:
- a) generating a vector having a selectable marker, a chemical sensor module and a regulated promoter;
- b) using the vector to assess the survivability of a bacterial strain;
- and
- c) selecting mutant libraries bacterial strain based on survivability, wherein the survivability is indicative of a genetic modification of the strain.
A method for enhanced production of a compound in a host cell comprising:
- a. optimizing a single expression regulatory sequence for producing a compound in the host cell utilizing at least two or more nucleic acid constructs;
- b. mobilizing the single expression regulatory sequence into a chemical sensor nucleic acid construct and transforming the host cell with the chemical sensor nucleic acid construct;
- c. screening for enhanced production of the compound in the host cell by providing a genomic library of predetermined sized genomic sequences, wherein the sequences are transformed in the host cell and one or more of the sequences are associated with the enhanced production;
- d. determining the host cell in (c) that provides the enhanced production;
- and
- e. culturing the host cell in (d) to provide the compound.
A host cell comprising a nucleic acid construct comprising a genetic element for increasing production of a compound in a host cell and a second expression regulatory element comprising a chemical sensor module, wherein the chemical sensor module is responsive to the compound.
A population of host cells comprising a nucleic acid construct comprising a genetic element that increases the production of a compound in a host cell, and a second nucleic acid construct comprising a chemical sensor module, wherein the chemical sensor module is responsive to the compound, and wherein the population comprises a genomic library of predetermined sized sequences.
Dependencies are inferred from the claim text.