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AssigneeMascoma Corporation
InventorsElena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
Estimating the term…
The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
An isolated polynucleotide comprising a nucleic acid fragment which encodes at least 50 contiguous amino acids of a mature cellulase, wherein said nucleic acid fragment is codon-optimized for expression in a yeast strain and wherein the cellulase is a termite cellulase or a termite-associated symbiont cellulase.
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A host cell comprising a polynucleotide encoding at least 50 contiguous amino acids of a mature heterologous cellulase, wherein the heterologous cellulase is a termite cellulase or a termite-associated symbiont cellulase, wherein the host cell is a yeast cell and wherein the heterologous cellulase is expressed.
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Dependencies are inferred from the claim text.