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OwnerJames Edward Hennessy, Amy Philbrook +6inventors
InventorsJames Edward Hennessy, Amy Philbrook, Daniel Miles Bartkus, Christopher John Easton, Colin Scott, John G. Oakeshott, Hye-Kyung Kim, Melissa Jane Latter
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The present invention relates to a method of producing carbamoyl phosphate, the method comprising reacting ammonia, ATP, bicarbonate and CO2, or a hydrated form thereof, in a composition in the presence of a carbamate kinase, wherein the ammonia and CO2, or hydrated form thereof, are converted to carbamate in a chemical reaction and the carbamate and ATP are converted to carbamoyl phosphate in an enzyme-catalysed reaction by the carbamate kinase, and wherein the pH of the composition is about 8 to about 12. The invention also relates to methods of producing urea.
A method of producing carbamoyl phosphate, the method comprising reacting ammonia, ATP, bicarbonate and CO2, or a hydrated form thereof, in a composition in the presence of a carbamate kinase, wherein the ammonia and CO2, or hydrated form thereof, are converted to carbamate in a chemical reaction and the carbamate and ATP are converted to carbamoyl phosphate in an enzyme-catalysed reaction by the carbamate kinase, and wherein the pH of the composition is about 8 to about 12.
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An isolated and/or exogenous polynucleotide encoding a carbamate kinase, wherein the polynucleotide comprises a sequence of nucleotides provided as any one of SEQ ID NOs:10, 12, 14, 16, 18, 20, 22, 24, 26 or 36 to 43.
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Dependencies are inferred from the claim text.