Means and methods of increasing viability of rod-shaped bacteria
AssigneeGeneral Mills, Inc.
InventorsBernhard H. Van Lengerich, Martin Senz, Ulf Stahl, Edeltraud Mast-Gerlach
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Abstract
This invention relates to use of peptone for controlling the volume and/or the length-to-diameter ratio of cells in culture, wherein said cells are cells of rod-shaped probiotic bacteria or rod-shaped fermentation bacteria.
Claims21
A method for controlling the volume and/or the length-to-diameter ratio of cells in culture, wherein said cells are cells of rod-shaped probiotic bacteria or rod-shaped fermentation bacteria, the method comprising exposing said cells to a peptone to result in reduced volume and/or length-to-diameter ratio of said cells.
A method comprising, culturing rod-shaped Gram positive bacteria in a cell culture medium, determining the average volume and/or the average length-to-diameter ratio of said cells in said culture medium, and selecting the culture medium as suitable for increasing viability, stability, or shelf life of the cells if the average volume of said cells is below 3 μm3 and/or the average length-to-diameter ratio of said cells is below 5.
A method of establishing a desired average volume and/or average length-to-diameter ratio of rod-shaped probiotic bacteria cells or rod-shaped fermentation bacteria cells, the desired average volume being below 3 μm3 and the desired average length-to-diameter ratio being below 5, the method comprising culturing said cells in the presence of fat stock peptone such that the cells reach the desired average volume and/or average length-to-diameter ratio.
A method of increasing viability, stability, shelf-life, DNA replication, septum formation and/or cell division of cells, wherein said cells are cells of rod-shaped probiotic bacteria or rod-shaped fermentation bacteria, the method comprising culturing said cells in the presence of fat stock peptone such that the volume of the cells reaches an average of less than 3 μm3 and/or the length-to-diameter ratio reaches an average of less than 5.
A method of preparing an extrudate, lyophilisate or frozen preparation comprising cells of rod-shaped probiotic bacteria or rod-shaped fermentation bacteria, said method comprising culturing said cells in the presence of fat stock peptone such that the cells reach an average volume of less than 3 μm3 and/or average length-to-diameter ratio of less than 5, extruding, lyophilising and/or freezing the cells.
A composition comprising rod-shaped probiotic bacteria and/or rod-shaped fermentation bacteria with an average volume below 3 μm3 and/or an average length-to-diameter ratio below 5.
(canceled)
A method of preparing a cell culture medium, said method comprising treating fat stock peptone in the presence of a reducing sugar at temperatures between 100° C. and 130° C. for at least 15 minutes.
Dependencies are inferred from the claim text.