Il-12 immunotherapy for cancer
AssigneeUniversity Health Network
InventorsJeffrey A. Medin, Christopher J. Paige
Life of this patent
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Abstract
Compositions and methods for delivering immune modulatory molecules to result in a therapeutic effect are disclosed. The compositions and methods use stably integrating lentiviral delivery systems. The methods are useful for therapeutically and prophylactically treating cancer such as leukemia.
Claims32
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A method of expressing IL-12 in a cell that is secreted above a threshold level, optionally a cancer cell, comprising:
- contacting the cell with a composition comprising a vector construct or an isolated virus comprising a lentiviral delivery vector and an IL-12 expression cassette, wherein the lentiviral delivery vector comprises a central polypurine tract (cPPT) sequence, optionally SEQ ID NO:
- 2 and/or a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), optionally SEQ ID NO:
- 3, under conditions that permit transduction of the cell, thereby providing a transduced cell, wherein the IL-12 is secreted and the IL-12 secreted per cell is above a threshold level.
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The method of claim 31, wherein the cancer is leukemia, optionally ALL, AML, CML or CLL.
A method of inducing or enhancing an immune response, optionally a memory immune response in a subject, optionally with cancer or an increased risk of cancer and/or treating a subject with cancer or an increased risk of cancer comprising administering:
- i) an isolated virus-comprising a lentiviral delivery vector and an IL-12 expression cassette wherein the lentiviral vector comprises a central polypurine tract (cPPT) sequence, optionally SEQ ID NO:
- 2 and/or a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), optionally SEQ ID NO:
- 3, ii) a transduced cell comprising a lentiviral delivery vector and an IL-12 expression cassette wherein the transduced cell expresses IL-12 above a threshold level iii) a population of cells comprising the transduced cell of ii), or iv) a composition comprising i), ii) and/or iii).
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A method of delivering IL-12 to a subject, optionally with cancer or an increased risk or cancer, optionally, for enhancing cancer treatment comprising:
- a. generating a recombinant IL-12 secreting cell wherein the IL-12 secreted per cell is above a threshold level;
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- b. introducing an effective number of the generated recombinant IL-12 secreting cells to the subject, optionally in a population of cells comprising the recombinant IL-12 secreting cell.
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Dependencies are inferred from the claim text.