Methods and compositions for detecting bk virus
AssigneeFocus Diagnostics, Inc.
InventorsFan Chen, Lilly I. Kong, Jules Chen, Mehrdad Jannatipour
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Abstract
The invention provides methods and compositions for rapid, sensitive, and highly specific nucleic acid-based (e.g., DNA based) detection of a BK virus in a sample. In general, the methods involve detecting a target nucleic acid having a target sequence of a conserved region of BK viral genomes. The invention also features compositions, including primers, probes, and kits, for use in the methods of the invention.
Claims31
1-30. (canceled)
A method of detecting the presence or absence of BK virus (BKV) nucleic acid in a sample, the method comprising contacting said sample with one or more oligonucleotides designed to hybridize to SEQ ID NO:
- 3 or a complement thereof but not to JC virus nucleic acid under conditions which discriminate between BKV and JCV nucleic acid and detecting if the hybridization of said one or more oligonucleotides to SEQ ID NO:
- 3 or a complement thereof has occurred, wherein said hybridization detects the presence or absence of BKV nucleic acid.
A method of detecting the presence or absence of BK virus (BKV) nucleic acid in a sample, the method comprising contacting said sample with one or more oligonucleotides designed to hybridize to SEQ ID NO:
- 4 or a complement thereof but not to JC virus nucleic acid under conditions which discriminate between BKV and JCV nucleic acid and detecting if the hybridization of said one or more oligonucleotides to SEQ ID NO:
- 4 or a complement thereof has occurred, wherein said hybridization detects the presence or absence of BKV nucleic acid.
Dependencies are inferred from the claim text.