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AssigneeCity of Hope
InventorsMarkus KALKUM, Karine BAGRAMYAN, Diana DIAZ-AREVALO, James I. ITO, Sanjeet DADWAL
Estimating the term…
Provided herein are methods for detecting an Aspergillus protease in a sample, diagnosing a subject with aspergillosis caused by an Aspergillus infection based on the presence of an Aspergillus protease in a sample, and methods of aspergillosis treatment that incorporate these diagnostic methods. In certain embodiments, the Aspergillus protease is Asp f2, and the Aspergillus infection is caused A. fumigatus, A. flavus, A. versicolor, A. niger, or A. terreus. Also provided herein are antibodies and kits for use in these methods, including novel antibodies specific for Asp f2.
A method for detecting Aspergillus Asp f2 in a biological sample comprising contacting the sample with an Asp f2 protease substrate comprising one or more Asp f2 cleavage sites, wherein cleavage of the protease substrate indicates the presence of Asp f2 in the sample.
A method for diagnosing aspergillosis in a subject comprising:
A method for the treatment of aspergillosis in a subject in need thereof comprising:
An Asp f2-specific antibody or antigen binding fragment thereof comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:1, 2, 8, 10, 12, 14, 16, 18, 20, and 22.
Dependencies are inferred from the claim text.