Differentiated cellular compositions and methods for their preparation and use
AssigneeLife Technologies Corporation
InventorsJonathan JACKSON, Stephen FERGUSON, Rafal WITEK
Life of this patent
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Abstract
The present invention relates to methods for culturing progenitor cells to differentiate primarily into a single population of cells with the same phenotype, and to compositions thereof. In particular, it relates to methods for culturing liver progenitor cells to differentiate primarily into a single population of hepatocyte-like cells, and to compositions thereof.
Claims19
A method for making a preparation substantially comprised of hepatocyte-like cells, the method comprising:
- inducing differentiation of hepatic progenitor cells and culturing the cells with an overlay comprising at least one extracellular matrix protein or fragment thereof.
An in vitro cell culture comprising a population of differentiated cells derived from a hepatic progenitor cell line, wherein population of differentiated cells comprises >70% hepatocyte-like cells.
A composition comprising
- an in vitro population of differentiated cells derived from a hepatic progenitor cell line, and
- a matrix overlay comprising at least one extracellular matrix protein or fragment thereof.
A differentiated population of HepaRG™ cells comprising >70% hepatocyte-like cells.
A method for increasing cytochrome P450 activity in an in vitro cell preparation, the method comprising:
- inducing differentiation of hepatic progenitor cells,
- culturing the cells with a matrix overlay for at least one day, the overlay comprising at least one extracellular matrix protein or fragment thereof, and
- measuring cytochrome P450 activity in the cell preparation, whereby the activity in the cell preparation is increased relative to a culture of the progenitor cells differentiated and cultured without a matrix overlay.
Dependencies are inferred from the claim text.