Methods, Systems, and Culture Medium for Production of Dechlorinating Microorganisms
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsRosa Krajmalnik-Brown, Cesar Torres, Anca Delgado, Sudeep Popat, Devyn Fajardo-Williams
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Abstract
Methods, systems, and compositions for growing high density cultures of dechlorinating microorganisms, such as the bacteria Dehalococcoides. Dechlorinating cultures are grown in continuous flow stirred-tank reactors at short hydraulic retention time, resulting in improved batch production. For some cultures, a culture medium including chlorine containing compounds, bicarbonate, and HEPES utilized.
Claims20
A method for dechlorination comprising:
- providing a culture medium including chlorinated compounds, to a continuous flow stirred-tank reactor;
- flowing the culture medium at achieve a specific hydraulic retention time;
- inoculating the culture medium with a culture adapted to dechlorinate the chlorinated compounds;
- culturing the culture in the presence of about 5 mM bicarbonate and HEPES;
- and
- converting the chlorinated compounds to ethene.
A system for dechlorination comprising:
- a continuous flow stirred-tank reactor;
- a culture medium in fluid communication with the continuous flow stirred-tank reactor wherein the culture medium comprises,
- chlorinated compounds;
- about 5 mM bicarbonate;
- and
- HEPES;
- a culture adapted to dechlorinate the chlorinated compounds;
- a peristaltic pump in fluid communication with both the continuous flow stirred-tank reactor and the culture medium;
- and
- an effluent collection bottle in fluid communication with the continuous flow stirred-tank reactor.
A composition for selectively growing high density cultures of Dehalococcoides comprising:
- a culture medium including,
- chlorinated compounds;
- about 2.5 mM to about 7.5 mM bicarbonate;
- and
- about 10 mM to about 25 mM HEPES.
Dependencies are inferred from the claim text.