Bacteria and method for synthesizing fatty acids
AssigneeWilliam Marsh Rice University
InventorsKa-Yiu San, Mai Li
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Abstract
The present invention discloses a process for increasing the production of free fatty acids at high yield (close to maximum theoretical yield), with various fatty acid compositions and various percentage of fatty acids accumulated intracellularly. This invention will enable the efficient production of other products derived from free fatty acids and/or products that can be branched out from the fatty acid synthesis pathways.
Claims20
A genetically engineered bacteria for producing fatty acids, said bacteria comprising a genotype comprising:
- b) an overexpressed acyl ACP thioesterase (TE+);
- c) one or more of an overexpressed FabZ+, FadR+, or FabH+;
- d) zero, one or more of a ΔfadR, ΔsucC, ΔfabR, ΔfadD, or ΔptsG;
- with the proviso that said bacteria does not have a genotype of ΔfadD ΔsucC TE+.
A genetically engineered bacteria for producing increased amounts of fatty acids, said bacteria comprising a genotype selected from the group consisting of:
- (1) ΔsucC fabZ+ acyl-ACP thioesterase+
- (2) ΔsucC ΔfabR fabZ+ acyl-ACP thioesterase+
- (3) fabZ+ acyl-ACP thioesterase+
- (4) fadR+ acyl-ACP thioesterase+
- (5) ΔsucC ΔfabR acyl-ACP thioesterase+
- (6) ΔfabR acyl-ACP thioesterase−
- (7) ΔfadR fabZ+ acyl-ACP thioesterase+
- (8) ΔfabR fabZ+ acyl-ACP thioesterase+
- (9) ΔfadD ΔsucC fabZ+ acyl-ACP thioesterase+
- (10) ΔfadD ΔsucC ΔfabR fabZ+ acyl-ACP thioesterase+
- (11) ΔfadD fabZ+ acyl-ACP thioesterase+
- (12) ΔfadD fadR+ acyl-ACP thioesterase+
- (13) ΔfadD ΔsucC ΔfabR acyl-ACP thioesterase+
- (14) ΔfadD ΔfabR acyl-ACP thioesterase+
- (15) ΔfadD ΔfadR fabZ+ acyl-ACP thioesterase+
- (16) ΔfadD ΔfabR fabZ+ acyl-ACP thioesterase+
- (17) ΔfadD ΔfabR fadR+ acyl-ACP thioesterase+
- (18) ΔfabR fadR+ acyl-ACP thioesterase−
- (19) ΔfadD ΔsucC ΔfabR fadR− acyl-ACP thioesterase+
- (20) ΔsucC ΔfabR fadR+ acyl-ACP thioesterase+
- (21) ΔfadD ΔptsG fabH+ acyl-ACP thioesterase+.
A genetically engineered Enterobacteriaceae comprising ΔfadD ΔsucC FabZ+ TE+.
A genetically engineered Enterobacteriaceae comprising ΔfadD ΔsucC ΔfabR FabZ+ TE+.
A genetically engineered Enterobacteriaceae comprising ΔfadD FabZ+ TE+.
A genetically engineered Enterobacteriaceae comprising ΔsucC FabZ+ TE+.
A genetically engineered Enterobacteriaceae comprising ΔsucC ΔfabR FabZ+ TE+.
A genetically engineered Enterobacteriaceae comprising FabZ+ TE+.
A method of producing fatty acids, comprising culturing a genetically engineered bacteria in a culture medium under conditions effective for the production of fatty acids;
- and harvesting said fatty acids from the microorganism and/or the culture medium, wherein the bacteria and the fatty acid profile are selected from the group consisting of those listed in Table 2.
Dependencies are inferred from the claim text.