Method and composition for staining and sample processing
AssigneeIris International, Inc.
InventorsJack Cremins, Carol Quon
Life of this patent
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Abstract
The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents, one or more permeabilizing agents, and one or more fixing agents. The particle contrast agent composition can include Crystal Violet, New Methylene Blue, Saponin, and Gluteraldehyde.
Claims13
A particle contrast agent composition for staining a blood fluid sample being imaged in an automated particle analysis system comprising:
- at least one particle contrast agent selected from the group consisting of Crystal Violet, New Methylene Blue, Methyl Green, Eosin Y, and Safranin O;
- a permeabilizing agent selected from the group consisting of a surfactant, a saponin, a quarternary ammonium salt, a nonionic surfactant, a detergent;
- and a zwitterionic surfactant;
- and
- a fixing agent selected from the group consisting of gluteraldehyde and formaldehyde.
A method of treating particles of a blood fluid sample which will be imaged using an automated particle analysis system comprising:
- combining the blood fluid sample with a particle contrast agent composition to obtain a sample mixture;
- and
- incubating the sample mixture at a temperature between about 37° Celsius and about 60° Celsius for fewer than 90 seconds;
- wherein the particle contrast agent composition includes:
- at least one particle contrast agent selected from the group consisting of Crystal Violet, New Methylene Blue, Methyl Green, Eosin Y, and Safranin O;
- a permeabilizing agent selected from the group consisting of a surfactant, a saponin, a quarternary ammonium salt, a nonionic surfactant, a detergent;
- and a zwitterionic surfactant;
- and
- a fixing agent selected from the group consisting of gluteraldehyde and formaldehyde.
Dependencies are inferred from the claim text.