Cell Pack for the Growth and Manipulation of Three Dimensional Cell Cultures
AssigneeThe Government of the United States of America, as represented by the Secretary of the Navy
InventorsThomas O'Shaughnessy, Paul T. Charles, Kirth Simmonds, Amit Bagchi, Ryan Mcculloch
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Abstract
Described herein is a sealed cell pack with a permeable membrane for growth and manipulation of three-dimensional cell cultures. This allows a cell culture to be removed from the laboratory and subjected to real world insults before being returned to culture conditions for continued growth and study. One application is for use in the study of the direct effects of blast waves on neuronal cells and methods for mitigating this response.
Claims19
A sealed cell pack comprising:
- a ring having an inner diameter, a thickness defined by distance between first and second opposing faces of the ring, and an interior volume defined by the thickness and the inner diameter of the ring;
- a porous membrane bonded to the first face of the ring, the porous membrane comprising pores sufficiently small to exclude passage of organisms and sufficiently large to permit passage of nutrients and waste;
- and
- a transparent film bonded to the second face of the ring,
- wherein the ring either (1) is comprised of elastomer or (2) includes a sealable orifice suitable for introduction of cells and growth media,
- wherein said interior volume is in a sterilized condition, and
- wherein the thickness of the ring is sufficiently small to allow diffusion of nutrients through the porous membrane to permit cell growth throughout said interior volume.
A sealed cell pack comprising:
- a ring having an inner diameter, a thickness defined by distance between first and second opposing faces of the ring, and an interior volume defined by the thickness and the inner diameter of the ring;
- a first porous membrane bonded to the first face of the ring;
- and
- a second porous membrane bonded to the second face of the ring;
- wherein the first and second porous membranes comprise pores sufficiently small to exclude passage of organisms and sufficiently large to permit passage of nutrients and waste,
- wherein the ring either (1) is comprised of elastomer or (2) includes a sealable orifice suitable for introduction is cells and growth media,
- wherein said interior volume is in a sterilized condition, and
- wherein the thickness of the ring is sufficiently small to allow diffusion of nutrients through the first and second porous membranes to permit cell growth throughout said interior volume.
A method of using a sealed cell pack, the method comprising:
- providing a sealed cell pack comprising
- a ring having an inner diameter, a thickness defined by distance between first and second opposing faces of the ring, and an interior volume defined by the thickness and the inner diameter of the ring;
- a porous membrane bonded to the first face of the ring, the porous membrane comprising pores sufficiently small to exclude passage of organisms and sufficiently large to permit passage of nutrients and waste;
- and
- a transparent film bonded to the second face of the ring,
- wherein the ring either (1) is comprised of elastomer or (2) includes a sealable orifice suitable for introduction of cells and growth media,
- wherein said interior volume is in a sterilized condition, and
- wherein the thickness of the ring is sufficiently small to allow diffusion of nutrients through the porous membrane to permit cell growth throughout said interior volume;
- and
- introducing living cells and a three-dimension matrix suitable for cellular growth into the interior volume.
The method of claim 18, further comprising visualizing said living cells through said transparent film while maintaining sterile culture conditions.
Dependencies are inferred from the claim text.