Microfluidic device

A method of microfluidic handling, the method comprising:

• filling a first cavity defined by a first layer with fluid through a channel;
• filling a second cavity defined by a second layer with fluid through a channel;
• providing a fluid separation between the first cavity and the second cavity;
• compressing the first and second layers relative to one another to cause sealing of the channels resulting in obstruction of the first cavity and the second cavity from further fluid entry;
• and
• removing the fluid separation and allowing for fluid communication between the first cavity and second cavity.

A microfluidic device, comprising:

• a first layer defining a first cavity;
• a first channel arranged to provide fluid entry to the first cavity;
• a second layer defining a second cavity;
• a second channel arranged to provide fluid entry to the second cavity;
• and
• at least one separating material providing fluid separation between the first cavity and the second cavity, wherein the first and second channels are adapted to be sealed resulting in obstruction of the first cavity and the second cavity from further fluid entry, and wherein heating or dissolving of the at least one separating material causes removal of the fluid separation allowing for fluid communication between the first cavity and second cavity.

A method of microfluidic handling, the method comprising:

• filling a first cavity defined by a first layer with fluid through a channel;
• filling a second cavity defined by a second layer with fluid through a channel;
• providing at least one separating material as a fluid separation between the first cavity and the second cavity;
• sealing the channels of the first and second layers resulting in obstruction of the first cavity and the second cavity from further fluid entry;
• and
• heating or dissolving the at least one separating material to remove the fluid separation and allowing for fluid communication between the first cavity and second cavity.