Method for detecting variation of gene for non-diagnostic purpose based on fluorescence quenching and probe thereof

A method for detecting variation of gene for non-diagnostic purpose based on fluorescence quenching, comprising steps of:

• 1) extracting DNA from a sample;
• 2) amplifying a gene segment to be detected based on the DNA;
• 3) performing an extension reaction with product of the step 2) as a template, probes, and ddNTP, and performing a fluorescence quenching reaction at the same time;
• wherein the probe is marked with quenching molecules, and the ddNTP is marked with fluorescent molecules;
• alternatively, the probe is marked with a fluorescence molecule, and the ddNTP is marked with a quenching molecule;
• 4) detecting changes of fluorescence caused by the extension reaction in the step 3), and calculating fluorescence quenching values;
• and
• 5) determining SNP of gene to be detected according to calculating results in the step 4).