Cd20 conformational isomers and methods of using
OwnerRoss Stewart Chambersinventor
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Abstract
Described herein are methods of screening for compounds that bind to conformational isomers of CD20 polypeptides. Also described are antibodies that bind to conformational isomers of CD20 polypeptides.
Claims19
A mutant CD20 nucleic acid, wherein said mutant CD20 nucleic acid has a sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5.
A method of screening for compounds that specifically bind to a conformational isomer of the CD20 polypeptide comprising:
- contacting a mutant CD20 polypeptide with a test compound, and
- determining whether or not the test compound binds to the mutant CD20 polypeptide,
- wherein binding of the test compound to the mutant CD20 polypeptide indicates a compound that binds to a conformational isomer of the CD20 polypeptide.
A method of screening for compounds that specifically bind to a conformational isomer of the CD20 polypeptide and to a wild type CD20 polypeptide comprising:
- contacting a mutant CD20 polypeptide with a test compound;
- contacting a wild type CD20 polypeptide with the test compound;
- and
- determining whether or not the test compound binds to both the mutant CD20 polypeptide and the wild type CD20 polypeptide,
- wherein binding of the test compound to both the mutant CD20 polypeptide and the wild type CD20 polypeptide indicates a compound that binds to both a conformational isomer of the CD20 polypeptide and to the wild type CD20 polypeptide.
A method of screening for compounds that specifically bind to a conformational isomer of the CD20 polypeptide and induce a conformational change comprising:
- contacting a mutant CD20 polypeptide with a test compound in the presence of an extracellular epitope-binding antibody;
- and
- determining whether or not the antibody binds to the mutant CD20 polypeptide in the presence of the test compound,
- wherein binding of the antibody to the mutant CD20 polypeptide in the presence of the test compound is indicative of a test compound that induces a conformational change in the conformational isomer of the CD20 polypeptide.
A method of making an antibody that specifically binds a conformational isomer of the CD20 polypeptide, comprising the steps of:
- immunizing a host animal with a mutant CD20 polypeptide.
Dependencies are inferred from the claim text.