Modified arsenite oxidase and a biosensor for detecting arsenite
AssigneeThe Bio Nano Centre Limited
InventorsAnthony Edward George Cass, Joanne Santini, Christopher James Johnson
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Abstract
The present invention provides an arsenite oxidase enzyme modified to prevent translocation by modification of a translocation signal sequence. A microorganism modified to express the heterologous arsenite oxidase enzyme is also provided by the invention, together with a device for detecting the presence of arsenite in a sample.
Claims24
An arsenite oxidase enzyme modified to prevent translocation to the periplasm, wherein the enzyme comprises the native AioA subunit from NT-26, or a variant, homologue or derivative thereof, and the native AioB subunit from NT-26, or a variant, homologue or derivative thereof, wherein a portion of the native AioB subunit corresponding to the translocation signal sequence, or a functional fragment thereof, is modified.
A modified enzyme according to any preceding claim, wherein the modification is deletion.
A modified enzyme according to any preceding claim, wherein the AioB subunit comprises of the peptide sequence identified as SEQ ID NO. 3, or a variant, homologue or derivative thereof, or is encoded by the polynucleotide sequence of SEQ ID NO. 7, or a variant, homologue or derivative thereof.
A modified enzyme according to any preceding claim, wherein the AioA subunit comprises the peptide sequence identified as SEQ ID NO. 4, or a variant, homologue or derivative thereof, or is encoded by the polynucleotide sequence of SEQ ID NO. 8, or a variant, homologue or derivative thereof.
Use of a modified arsenite oxidase enzyme according to any preceding claim in a biosensor to detect the presence of arsenite in a sample.
A device for detecting the presence of arsenite in a sample, comprising at least one electrode, an arsenite oxidase enzyme and a redox mediator.
Dependencies are inferred from the claim text.