Convenient and efficient purification method for chemically labeled nucleic acids
AssigneeSnu R&Db Foundation
InventorsSeong Keun Kim, Young Gyu Kim, Ji Hee Hwang
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AssigneeSnu R&Db Foundation
InventorsSeong Keun Kim, Young Gyu Kim, Ji Hee Hwang
Estimating the term…
The present invention relates to a method for purifying chemically labeled nucleic acids. More particularly, the present invention relates to a convenient and efficient method for purifying labeled nucleic acids in a fast manner with high efficiency from a mixture of unreacted hydrophobic probes, unreacted nucleic acids and labeled nucleic acids. In particular, the present invention relates to a convenient method for purifying labeled nucleic acids in a fast manner with high efficiency from a mixture of unreacted hydrophobic chemical probes, unreacted nucleic acids and labeled nucleic acids, comprising the steps of reacting hydrophobic chemical probe and nucleic acids in an aqueous solution to prepare a reacted solution; adding organic solvent to the reacted solution and stirring it to obtain a mixed solution; and centrifuging the mixed solution and removing organic solvent phase.
A purification method for chemically labeled nucleic acids comprising:
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