Methods for preparing single domain antibody microarrays

A single domain antibody (sdAb) microarray obtained by

• i) providing a host cell capable of expressing a biotinylation enzyme
• ii) transforming said host cell with a nucleic acid encoding for a fusion protein comprising a sdAb fused at its carboxy terminal end to a biotinylation peptide
• iii) culturing said host cell in presence of biotin in such a way that said fusion protein is expressed, resulting in biotinylation of said fusion protein
• iv) lysing said host cell as cultured at step iii) and
• v) spotting the Mate obtained at step iv) on a solid support coated with an agent selected from the group consisting of avidin, streptavidin and/or a derivative of avidin or streptavidin.

A method for detecting a plurality of antigens comprising the steps of

• providing a single domain antibody (sdAb) array by
• i) providing a host cell capable of expressing a biotinylation enzyme
• ii) transforming said host cell with a nucleic acid encoding for a fusion protein comprising a sdAb fused at its carboxy terminal end to a biotinylation peptide
• iii) culturing said host cell in presence of biotin in such a way that said fusion protein is expressed, resulting in biotinylation of said fusion protein
• iv) lysing said host cell as cultured at step iii) and
• v) spotting the lysate obtained at step iv) on a solid support coated with an agent selected from the group consisting of avidin, streptavidin and/or a derivative of avidin or streptavidin,
• contacting the array with a sample containing antigens, and
• detecting antigens that bind to the array.