US 2014/0235478 A1 · Measurement and Comparison of Immune Diversity by High-Throughput Sequencing

Abstract

High-throughput long read sequencing is used to perform immunogenomic characterization of expressed antibody repertoires in the context of vaccination. Informatic analysis allows global characterizations of isotype distributions, determination of the lineage structure of the repertoire and measure age and antigen related mutational activity. Global analysis of the immune system's clonal structure provides direct insight into the effects of vaccination and provides a detailed molecular portrait of age-related effects.

Claims11

Dependent claims

A method of characterizing an immune response to administration of a vaccine, comprising:

obtaining nucleic acid from a biological sample;
sequencing nucleic acid encoding at least a portion of a subject's immune repertoire;
and
comparing sequences obtained to known sequences associated with immune function, thereby to characterize the subject's immune repertoire.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

14/172,642Filed 4 Feb 2014

Priority

4 Feb 2013

Published

21 Aug 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material
C12Q 2600/106
Oligonucleotides characterized by their use: pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
C12Q 2600/156
Oligonucleotides characterized by their use: polymorphic or mutational markers
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

Measurement and Comparison of Immune Diversity by High-Throughput Sequencing

Life of this patent

Measurement and Comparison of Immune Diversity by High-Throughput Sequencing

Life of this patent

Abstract

Claims11