Use of gene regulatory network logic for transformation of cells
AssigneeCalifornia Institute of Technology
InventorsIsabelle Peter, Yong Zhu, Eric H. Davidson
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Abstract
Described herein is a gene regulatory network based focused approach to cell transformation. The methods described herein allow for identification of circuit and sub-circuit repertoires for which modification in a starting cell type can result in generation of a transformed cell type in a durable and persistent manner, without requiring potentially deleterious genome modification. The described methods and compositions produced by the methods find widespread application in regenerative medicine applications.
Claims27
A method of transforming a cell, comprising:
- providing a quantity of at least one cis regulatory network element;
- and
- introducing into a starting cell type, the at least one cis regulatory network element, wherein the at least one cis regulatory network element is capable of altering a regulatory sub-circuit in the starting cell type, thereby altering one of more properties of the starting cell type, and generating a transformed cell type.
A method for identifying a regulatory network for transforming a cell, comprising:
- organizing a plurality of cis regulatory network elements into a network topology of nodes comprising circuits, wherein the circuits comprise at least one sub-circuit;
- and
- identifying at least one sub-circuit comprising at least one positive effector node, wherein the at least one positive effector node is capable of generating a transformed cell type when introduced into a staring cell.
A composition comprising:
- a quantity of cells expressing at least one exogenously added protein, wherein the at least one exogenously added protein is a positive effector in a regulatory sub-circuit.
Dependencies are inferred from the claim text.