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US 2014/0234855 A1Published application
Chromophore compositions and methods of making and using the same
AssigneeBecton, Dickinson and Company
InventorsMajid Mehrpouyan, Oleg Guryev, Marybeth Sharkey
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Abstract
Chromophore compositions and methods of making and using the same are provided. Aspects of the chromophore compositions include a chromophore component having a chromophore, such as a fluorescent dye moiety, stably associated with a prosthetic group binding cavity of a metalloprotein. Also provided are methods of making, methods of use, systems and kits related to the subject fluorescent compositions.
Claims20
A fluorescent composition, the composition comprising:
- a fluorescent component comprising:
- (i) a metalloprotein comprising a prosthetic group binding cavity;
- and
- (ii) a fluorescent dye moiety stably associated with the prosthetic group binding cavity;
- wherein the metalloprotein comprises a stabilizing modification that enhances association of the dye moiety with the cavity.
2 more elements
A fluorescent composition, the composition comprising:
- a fluorescent component comprising:
- (i) a metalloprotein comprising a prosthetic group binding cavity;
- and
- (ii) an environmentally sensitive fluorescent dye moiety stably associated with the prosthetic group binding cavity.
1 more element
A method of assaying a sample, the method comprising:
- contacting the sample with a fluorescent composition, wherein the fluorescent composition comprises:
- i) a metalloprotein comprising a prosthetic group binding cavity;
- ii) a fluorescent dye moiety stably associated with the prosthetic group binding cavity.
A kit comprising:
- a fluorescent composition, the fluorescent composition comprising:
- (i) a metalloprotein comprising a prosthetic group binding cavity;
- and
- (ii) a fluorescent dye moiety stably associated with the prosthetic group binding cavity;
- and
- a container comprising the fluorescent composition.
3 more elements
Dependencies are inferred from the claim text.