2-hydroxyhippuric acid analogs, and methods for their synthesis and use
AssigneeAlere San Diego, Inc.
InventorMariusz Banaszczyk
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Abstract
The present invention relates to novel 2-hydroxyhippuric acid analogs, and methods for their synthesis and use. Such analogs are designed to provide a protected or functional moiety such as a free thiol (—SH) group or a protected thiol group, thereby providing a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.
Claims26
A compound or salt thereof, said compound having the structure:
- Wherein R1 is a linking group having the structure
- where
- W is C0-12 unsubstituted, substituted, liner or branched alkyl;
- X is an optionally present C(O);
- Y is an optionally substituted C0-12 alkyl or N(H)—C0-6 alkyl, and is optionally present;
- and
- Z is a functional moiety selected from the group consisting of protected or unprotected sulfhydryl moieties, protected or unprotected amine moieties, primary amine-reactive moieties, sulfhydryl-reactive moieties, photoreactive moieties, carboxyl-reactive moieties, arginine-reactive moieties, and carbonyl-reactive moieties.
A compound or salt thereof, said compound having the structure:
- Wherein R1 is a linking group having the structure
- where
- W is C0-12 unsubstituted, substituted, liner or branched alkyl;
- X is an optionally present C(O);
- Y is an optionally substituted C0-12 alkyl or N(H)—C0-6 alkyl, and is optionally present;
- and
- Z is a moiety providing a thiol or a protected thiol at its terminus.
A method of determining a 2-hydroxyhippuric acid concentration in a sample, comprising:
- performing a competitive immunoassay in which a conjugate having the structure
- wherein R2 is a linkage chemistry and P is a detectable label, wherein R2 and P are covalently linked, and wherein the conjugate competes with 2-hydroxyhippuric acid in said sample for binding to an antibody, wherein a signal obtained from said assay is indicative of the concentration of 2-hydroxyhippuric acid in said sample;
- and
- determining the concentration of 2-hydroxyhippuric acid in said sample from the assay signal.
A method of determining a 2-hydroxyhippuric acid concentration in a sample, comprising:
- performing a competitive immunoassay in which 2-hydroxyhippuric acid in said sample competes for binding to an antibody raised against an immunogen having the structure
- wherein R2 is a linkage chemistry and P is a carrier polypeptide, wherein R2 and P are covalently linked, wherein a signal obtained from said assay is indicative of the concentration of 2-hydroxyhippuric acid in said sample;
- and
- determining the concentration of 2-hydroxyhippuric acid in said sample from the assay signal.
Dependencies are inferred from the claim text.